RPA70 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91179-50, BS91179-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P27694(Human)
Host: Rabbit
Reactivity: Human
Applications: WB, ICC IF FC
All Applications: WB:1:500
ICC:1:50-1:100
FC:1:50-1:100
Background: The single-stranded-DNA-binding proteins (SSBs) are essential for DNA function in prokaryotic and eukaryotic cells, mitochondria, phages and viruses. Replication protein A (RPA), a highly conserved eukaryotic protein, is a heterotrimeric SSB. RPA plays an important role in DNA replication, recombination and repair. The binding of human RPA (hRPA) to DNA involves molecular polarity in which initial hRPA binding occurs on the 5' side of an ssDNA substrate and then extends in the 3' direction to create a stably bound hRPA. RPA is a major damage-recognition protein involved in the early stages of nucleotide excision repair. It can also play a role in telomere maintenance. The RPA 70 kDa subunit binds to ssDNA and mediates interactions with many cellular and viral proteins. The DNA binding domain lies in the middle of RPA 70 kDa subunit and comprises two structurally homologous subdomains oriented in tandem. RPA contains a conserved four cysteine-type zinc-finger motif, which mediates the transition of RPA-ssDNA interaction to a stable RPA-ssDNA complex in a redox-dependent manner.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: RPA70 polyclonal antibody detects endogenous levels of RPA70 protein.
Bioworld Molecular Weight: 68 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of RPA70 on Siha cell lysates using anti-RPA70 antibody at 1/500 dilution., ICC staining RPA70 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: RP-A p70, RF-A protein 1, RPA1, REPA1, RPA70, Dmrpa1, Drosophila Replication Protein A, DRPA, HSSB, Human single stranded DNA binding protein, MST075, MSTP075, p70, REPA1, Replication factor A, Replication factor A protein 1, Replication protein A 70 kDa DNA-binding subunit, Replication protein A 70kDa DNA binding subunit, Replication protein A1 70kDa, Replication protein A1, RF A, RF-A protein 1, RFA, RFA1, RP A, RP-A p70, RPA 70, RPA, rpa1, Single stranded binding protein 70, Single-stranded DNA-binding protein,
Conjugate: Unconjugated
Modification: Unmodified