Rpb1 (phospho-S1619) Polyclonal Antibody-BS4246

Rpb1 (phospho-S1619) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS4246-50, BS4246-100

Swiss-Prot: P24928

Host: Rabbit

Rpb1 (phospho-S1619) Polyclonal Antibody-BS4246

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: RPB1 is a subunit of RNA polymerase II which catalyses transcription of RNA from DNA. RPB1 forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-Rpb1 (S1619) polyclonal antibody detects endogenous levels of Rpb1 protein only when phosphorylated at Ser1619.

Bioworld Molecular Weight: ~ 280 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of p-Rpb1 (S1619) pAb at 1:2000 dilution
Lane 1: A549 whole cell lysate (40ug)
Lane 2: Hela whole cell lysate (40ug)
Lane 3: A2780 whole cell lysate (40ug)
Lane 4: PC12 whole cell lysate (40ug)
Lane 5: 3T3-L1 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of p-Rpb1 (S1619) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Alternative Name: DNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA-directed RNA polymerase II subunit RPB1; POLR2A; POLR2

Immunogen: Synthetic phosphopeptide derived from human Rpb1 around the phosphorylation site of Serine 1619.

Modification: Phosphorylation

Conjugate: Unconjugated