RPB3/POLR2C Polyclonal Antibody-BS7292

RPB3/POLR2C Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS7292-50, BS7292-100

Swiss-Prot: P19387

Host: Rabbit

RPB3/POLR2C Polyclonal Antibody-BS7292

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse

Applications: WB, IP,

All Applications: WB,1:500 - 1:2000 | IP,1:20 - 1:50

Background: This gene encodes the third largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a cysteine rich region and exists as a heterodimer with another polymerase subunit, POLR2J. These two subunits form a core subassembly unit of the polymerase. A pseudogene has been identified on chromosome 21.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 38kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using RPB3/POLR2C antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST., Immunoprecipitation analysis of 150ug extracts of SW620 cells using 3ug RPB3/POLR2C antibody. Western blot was performed from the immunoprecipitate using RPB3/POLR2C antibody at a dilution of 1:500.

Alternative Name: POLR2C; RPB3; RPB31; hRPB33; hsRPB3

Immunogen: Recombinant fusion protein of human RPB3/POLR2C(NP_116558.1).

Modification: Unmodified

Conjugate: Unconjugated