Product Description

RPL27A (V140) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3049-50, BS3049-100

Sizes: 50µl, 100µl

Swiss-Prot: P46776

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Ribosomes, the organelles that catalyze protein synthesis, are composed of a small subunit (40S) and a large subunit (60S) that consist of over 80 distinct ribosomal proteins. Mammalian ribosomal proteins are encoded by multigene families that contain processed pseudogenes and one functional intron-containing gene within their coding regions. Ribosomal Protein L27, also known as RPL27, is a 136 amino acid protein belonging to the ribosomal protein L27e family exists as a component of the 60S subunit, possibly playing a role in protein translation. Like most ribosomal proteins, Ribosomal Protein L27 exists as multiple processed pseudogenes that are scattered throughout the genome. Considered a novel candidate housekeeping gene, the gene encoding Ribosomal Protein L27 maps to human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Ribosomal Protein L27A (V140) polyclonal antibody detects endogenous levels of Ribosomal Protein L27A protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of RPL27A (V140) pAb at 1:500 dilution
Lane 1: Hela whole cell lysate (40ug)
Lane 2: MCF-7 whole cell lysate (40ug)
Lane 3: The Brain tissue lysate of Mouse (40ug)
Lane 4: The Brain tissue lysate of Rat (40ug), Immunohistochemistry (IHC) analyzes of RPL27A (V140) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 17 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: 60S ribosomal protein L27a

Immunogen: Synthetic peptide, corresponding to amino acids 120-160 of Human Ribosomal Protein L27A.

Conjugate: Unconjugated

Modification: Unmodified