Product Description

RPL3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65387-50, BS65387-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Swiss-Prot: P39023

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-P, IF (paraffin section), ELISA

All Applications: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

Background: ribosomal protein L3(RPL3) Homo sapiens Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L3P family of ribosomal proteins and it is located in the cytoplasm. The protein can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. This gene is co-transcribed with several small nucleolar RNA genes, which are located in several of this gene's introns. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: RPL3 Polyclonal Antibody detects endogenous levels of RPL3 protein.

Bioworld Molecular Weight: ~ 46 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot analysis of various cells using Ribosomal Protein L3 Polyclonal Antibody, Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using RPL3 Antibody. The picture on the right is blocked with the synthesized peptide., Western blot analysis of lysates from HT-29 cells, using RPL3 Antibody. The Lane on the right is blocked with the synthesized peptide., Western blot analysis of the lysates from HUVECcells using RPL3 antibody.

Alternative Name: RPL3; OK/SW-cl.32; 60S ribosomal protein L3; HIV-1 TAR RNA-binding protein B; TARBP-B

Immunogen: The antiserum was produced against synthesized peptide derived from human RPL3. AA range:211-260

Conjugate: Unconjugated

Modification: Unmodified