Product Description

Ephrin-B1/2 (Q324) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS1398-50, BS1398-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P98172/P52799

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IHC

All Applications: IHC: 1:50~1:200

Background: Ephrin-B1 transduces outside-in signals through C-terminal protein interactions that effect integrin-mediated cell attachment and migration. The distribution of ephrin-B1 in the developing retina suggests that it influences retinal axon mapping along the dorsal-ventral axis and may be involved in intratectal development The transmembrane ligand ephrin-B2 and its receptor tyrosine kinase EphB4 are specifically expressed on arterial and venous endothelial cells, respectively. Bidirectional signals mediated by both proteins play an important role in vascular development. Ephrin-B2 is essential for the normal morphogenesis of the embryonic vasculature and is angiogenic in tumors. It has been identified as an important target of chemotherapeutic treatments.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry (IHC) analyzes of Ephrin-B1/2 (Q324) pAb in paraffin-embedded human esophageal carcinoma tissue at 1:50. showing cytoplasmic staining. Negative control (the right) Using PBS instead of primary antibody,Secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 38, 45, 59 kDa

Specificity: Ephrin-B (Q324) polyclonal antibody detects endogenous levels of Ephrin-B protein.

Alternative Name: Ephrin B1; EphrinB1; EFL-3; ELK ligand; ELK-L; EPH-related receptor tyrosine kinase ligand 2; LERK-2; EFNB1; EFL3; EPLG2; LERK2; Ephrin-B2; EPH-related receptor tyrosine kinase ligand 5; LERK-5; HTK ligand; HTK-L; EFNB2; EPLG5; HTKL; LERK5

Immunogen: Synthetic peptide, corresponding to amino acids 300-340 of Human Ephrin-B1.

Conjugate: Unconjugated

Modification: Unmodified