Product Description

RPP30 Polyclonal Antibody

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS71248-50, BS71248-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P78346

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF/ICC

All Applications: WB,1:500 - 1:2000 | IHC,1:100 - 1:200 | IF/ICC,1:50 - 1:200

Background: Component of ribonuclease P, a ribonucleoprotein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 29kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using RPP30 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Immunohistochemistry of paraffin-embedded human lung cancer using RPP30 antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol.

Alternative Name: RPP30; TSG15

Immunogen: Recombinant fusion protein of human RPP30(NP_006404.1).

Conjugate: Unconjugated

Modification: Unmodified