
RPRD1B Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS72306-50, BS72306-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q9NQG5
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
All Applications: WB,1:500 - 1:2000 | IHC,1:100 - 1:200
Background: Interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD by RPAP2. Transcriptional regulator which enhances expression of CCND1. Promotes binding of RNA polymerase II to the CCDN1 promoter and to the termination region before the poly-A site but decreases its binding after the poly-A site. Prevents RNA polymerase II from reading through the 3' end termination site and may allow it to be recruited back to the promoter through promotion of the formation of a chromatin loop. Also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. Promotes cell proliferation.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Polyclonal Antibodies
Bioworld Molecular Weight: 37kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western Blot analysis of extracts of various cell lines, using rprd1b antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Alternative Name: RPRD1B; C20orf77; CREPT; NET60; dJ1057B20.2; K-H; Kub5-Hera; rprd1b
Immunogen: Recombinant fusion protein of human rprd1b(Q9NQG5).
Conjugate: Unconjugated
Modification: Unmodified