Product Description

RPS6 (L234) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS1592-50, BS1592-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregμlating mRNA translation. Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions. These particμlar mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation. Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein.

Category: Primary Antibody

Reactivity: Human, Rat, Mouse

Host: Rabbit

Applications: WB, IP

Alternate Names: 40S ribosomal protein S6, Phosphoprotein NP33, Small ribosomal subunit protein Es6, RPS6, OK/SW-cl.2

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to Human RPS6.

Dilution: WB: 1:5000~1:10000
IP: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: RPS6 (L234) polyclonal antibody detects endogenous levels of RPS6 protein.

Molecular Weight: ~ 32 kDa

Pathway: MAPK Erk in Growth and Differentiation Pathway(, PI3K AKT signaling, Translational Contral elF4 and p70 S6 Kinase,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P62753

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only