Product Description

RRM1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS75303-50, BS75303-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P23921

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IF/ICC, IP,

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200 | IP,1:20 - 1:50

Background: This gene encodes the large and catalytic subunit of ribonucleotide reductase, an enzyme essential for the conversion of ribonucleotides into deoxyribonucleotides. A pool of available deoxyribonucleotides is important for DNA replication during S phase of the cell cycle as well as multiple DNA repair processes. Alternative splicing results in multiple transcript variants.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 95kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using RRM1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST., Immunofluorescence analysis of HeLa cells using RRM1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining., Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug RRM1 antibody. Western blot was performed from the immunoprecipitate using RRM1 antibody at a dilution of 1:500.

Alternative Name: RRM1; R1; RIR1; RR1

Immunogen: Recombinant fusion protein of human RRM1(NP_001024.1).

Conjugate: Unconjugated

Modification: Unmodified