Product Description

RRN3 (phospho-S649) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS64367-50, BS64367-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: In eukaryotes, ribosomal RNA genes are transcribed by RNA polymerase (pol I). In Saccharomyces cerevisiae, transcription of rRNA genes requires at least three transcription factors, which include the two mμltisubunit factors Core factor and UAF that function in the assembly of the preinitiation complex. The third factor, Rrn3, functions as a single subunit and is not required for the preinitiation complex assembly. Unlike other pol I transcription factors, Rrn3 is functionally conserved between yeast and mammals as an rRNA gene transcription regμlator. Human Rrn3 is 21% homologous to the yeast Rrn3 protein and is a member of a conserved gene family spanning the fungi, plant and animal kingdoms. hRrn3 is highly expressed in the lung, retina, thymus, and prostate. Rrn3 may be identical to the transcription factor TIF-IA, since both TIF-IA and Rrn3 associate with pol I and their activities are growth rate dependent.

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB

Alternate Names: RNA polymerase I-specific transcription initiation factor RRN3, Transcription initiation factor IA, TIF-IA, RRN3, TIFIA

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human RRN3 around the phosphorylation site of Serine649.

Dilution: WB: 1:500~1:1000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: RRN3 (phospho-S649) polyclonal antibody detects endogenous levels of RRN3 protein only when phosphorylated at Ser649 .

Molecular Weight: ~ 80 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q9NYV6

Product: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.

Research Use Only