Product Description

RSPO3 Polyclonal Antibody

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS71861-50, BS71861-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9BXY4

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: This gene belongs to the R-spondin family. The encoded protein plays a role in the regulation of Wnt (wingless-type MMt V integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis. Genome-wide association studies suggest a correlation of this gene with bone mineral density and risk of fracture. This gene may be involved in tumor development.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using RSPO3 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.

Bioworld Molecular Weight: 39kDa

Specificity: Polyclonal Antibodies

Alternative Name: RSPO3; CRISTIN1; PWTSR; THSD2

Immunogen: A synthetic peptide of human RSPO3(NP_116173.2).

Conjugate: Unconjugated

Modification: Unmodified