RTCD1 Polyclonal Antibody-BS5918

RTCD1 Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS5918-50, BS5918-100

Sizes: 50µl, 100µl

Swiss-Prot: O00442

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, IHC

Background: RTCD1 catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3'P to produce RNA-N3'PP5'A; (C) a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing. There are two named isoforms.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: RTCD1 polyclonal antibody detects endogenous levels of RTCD1 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of RTCA pAb at 1:500 dilution
Lane 1: SGC7901 whole cell lysate (40ug)
Lane 2: Panc1 whole cell lysate (40ug)
Lane 3: C6 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of RTCD1 pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 43 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: RNA 3'-terminal phosphate cyclase; RNA cyclase; RNA-3'-phosphate cyclase; RNA terminal phosphate cyclase domain-containing protein 1; RTC domain-containing protein 1; RTCA; RPC; RPC1; RTC1

Immunogen: Synthetic peptide, corresponding to amino acids 321-366 of Human RTCD1.

Conjugate: Unconjugated

Modification: Unmodified