Rubicon Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS75217-50, BS75217-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q92622
Host: Rabbit
Reactivity: Mouse
Applications: WB
All Applications: WB,1:500 - 1:2000
Background: The protein encoded by this gene is a negative regulator of autophagy and endocytic trafficking and controls endosome maturation. This protein contains two conserved domains, an N-terminal RUN domain and a C-terminal DUF4206 domain. The RUN domain is involved in Ras-like GTPase signaling, and the DUF4206 domain contains a diacylglycerol (DAG) binding-like motif. Mutation in this gene results in deletion of the DAG binding-like motif and causes a recessive ataxia. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Unmodification
Extra Notes: Western blot analysis of extracts of mouse brain, using Rubicon antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Note: For research use only, not for use in diagnostic procedure.
Bioworld Molecular Weight: 108kDa
Alternative Name: RUBCN; KIAA0226; RUBICON; SCAR15
Immunogen: Recombinant fusion protein of human Rubicon(NP_055502.1).
Conjugate: Unconjugated
Modification: Unmodified