Product Description

RUNX1T1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS77245-50, BS77245-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q06455

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB,1:200 - 1:1000

Background: This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Alternative splicing results in multiple transcript variants.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Extra Notes: Western blot analysis of Rat brain, using RUNX1T1 antibody at 1:690 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

Note: For research use only, not for use in diagnostic procedure.

Bioworld Molecular Weight: 68KDa

Alternative Name: RUNX1T1; AML1-MTG8; AML1T1; CBFA2T1; CDR; ETO; MTG8; ZMYND2

Immunogen: Recombinant fusion protein of human RUNX1T1(NP_783554.1).

Conjugate: Unconjugated

Modification: Unmodified