Product Description

RUNX2 Polyclonal Antibody

Catalogue Numbers:BS91188-50, BS91188-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13950(Human) Q08775(Mouse) Q9Z2J9(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: ICC/IF, IHC, WB

All Applications: WB:1:1,000
ICC:1:50-1:200
IHC:1:50-1:200

Background: The mammalian Runt-related transcription factor (RUNX) family comprises three members, RUNX1 (also designated AML-1, PEBP2αB, CBFA2), RUNX2 (also designated AML-3, PEBP2αA, CBFA1, Osf2) and RUNX3 (also designated AML-2, PEBPαC, CBFA3). RUNX family members are DNA-binding proteins that regulate the expression of genes involved in cellular differentiation and cell cycle progression. RUNX2 is essential for skeletal mineralization in that it stimulates osteoblast differentiation of mesenchymal stem cells, promotes chondrocyte hypertrophy and contributes to endothelial cell migration and vascular invasion of developing bones. Regulating RUNX2 expression may be a useful therapeutic tool for promoting bone formation. Mutations in the C-terminus of RUNX2 are associated with cleidocranial dysplasia syndrome, an autosomal-dominant skeletal dysplasia syndrome that is characterized by widely patent calvarial sutures, clavicular hypoplasia, supernumerary teeth, and short stature.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: RUNX2 polyclonal antibody detects endogenous levels of RUNX2 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: ICC staining RUNX2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-RUNX2 antibody. Counter stained with hematoxylin.

Alternative Name: CBF-alpha-1, OSF-2, PEA2-alpha A, PEBP2-alpha A, RUNX2, Acute myeloid leukemia 3 protein, Alpha subunit 1, AML3, CBF alpha 1, CBF-alpha-1, CBFA1, CCD, CCD1, Cleidocranial dysplasia 1, Core binding factor, Core binding factor runt domain alpha subunit 1, Core binding factor subunit alpha 1, Core-binding factor subunit alpha-1, MGC120022, MGC120023, Oncogene AML 3, Oncogene AML-3, OSF 2, OSF-2, OSF2, Osteoblast specific transcription factor 2, Osteoblast-specific transcription factor 2, OTTHUMP00000016533, PEA2 alpha A, PEA2-alpha A, PEA2aA, PEBP2 alpha A, PEBP2-alpha A, PEBP2A1, PEBP2A2, PEBP2aA, PEBP2aA1, Polyomavirus enhancer binding protein 2 alpha A subunit, Polyomavirus enhancer-binding protein 2 alpha A subunit, Runt domain, Runt related transcription factor 2, Runt-related transcription factor 2, RUNX2, RUNX2, SL3 3 enhancer factor 1 alpha A subunit, SL3-3 enhancer factor 1 alpha A subunit, SL3/AKV core binding factor alpha A subunit, SL3/AKV core-binding factor alpha A subunit,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified