S100-A10 Monoclonal Antibody-MB66608

S100-A10 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB66608-50, MB66608-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P60903

Host: Mouse

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC, IP,

All Applications: WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100), IP (1/10 - 1/50)

Background: Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine-specific kinase.Does not appear to bind calcium. Contains 2 ancestral calcium site related to EF-hand domains that have lost their ability to bind calcium.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of S100-A10 protein.

Bioworld Molecular Weight: ~ 13 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of S100-A10 expression in C6 (A), NIH3T3 (B), Hela (C) whole cell lysates., Immunofluorescent analysis of S100-A10 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Alternative Name: ANX2LG; CAL1L; CLP11; Protein S100-A10; Calpactin I light chain; Calpactin-1 light chain; Cellular ligand of annexin II; S100 calcium-binding protein A10; p10 protein; p11

Immunogen: Purified recombinant mouse S100A10 protein fragments expressed in E.coli.

Conjugate: Unconjugated

Modification: Unmodified