Product Description

S100-A4 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66609-50, MB66609-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Despite their relatively small size (8-12 kDa) and uncomplicated architecture, S100 proteins regμlate a variety of cellμlar processes, such as cell growth and motility, cell cycle progression, transcription, and differentiation. To date, 25 members have been identified, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z, making it the largest group in the EF-hand, calcium-binding protein family. Interestingly, 14 S100 genes are clustered on human chromosome 1q21, a region of genomic instability. Research studies have demonstrated that significant correlation exists between aberrant S100 protein expression and cancer progression. S100 proteins primarily mediate immune responses in various tissue types but are also involved in neuronal development. Each S100 monomer bears two EF-hand motifs and can bind up to two molecμles of calcium (or other divalent cation in some instances). Structural evidence shows that S100 proteins form antiparallel homo- or heterodimers that coordinate binding partner proximity in a calcium-dependent (and sometimes calcium-independent) manner. Although structurally and functionally similar, individual members show restricted tissue distribution, are localized in specific cellμlar compartments, and display unique protein binding partners, which suggests that each plays a specific role in various signaling pathways. In addition to an intracellμlar role, some S100 proteins have been shown to act as receptors for extracellμlar ligands or are secreted and exhibit cytokine-like activities. Research studies have shown that S100A4 is overexpressed in highly metastatic cancers, which makes it usefμl as a marker of tumor progression and may serve as a prognostic factor in several cancer types. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, nonmuscle myosin IIA, α6β4 integrin, and liprin b1. S100A4 is present in the nucleus, cytoplasm and extracellμlar space. Intracellμlar and extracellμlar S100A4 both promote cell migration via interaction with different proteins. Researchers have recently discovered that S100A4 also functions as a neuroprotectant in the peripheral nervous system.

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB, IP

Alternate Names: CAPL, MTS1, Protein S100-A4, Calvascμlin, Metastasin, Placental calcium-binding protein, Protein Mts1, S100 calcium-binding protein A4

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant human S100A4 protein.

Dilution: WB (1/500 - 1/1000), IP (1/10 - 1/50)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of S100-A4 protein.

Molecular Weight: ~ 12 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P26447

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Research Use Only