Product Description

S100 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91192-50, BS91192-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P23297(Human) P56565(Mouse)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IP, IHC FC

All Applications: WB:1:500-1:2,000
IHC:1:50-1:200
FC:1:50-1:100
IP:1:10-1:50

Background: The family of EF-hand type Ca2+-binding proteins includes calbindin (previously designated vitamin D-dependent Ca2+-binding protein), S-100 α and β, calgranulins A (also designated MRP8), B (also designated MRP14) and C (S-100 like proteins), and the parvalbumin family members, including parvalbumin α and parvalbumin β (also designated oncomodulin). The S-100 protein is involved in the regulation of cellular processes such as cell cycle progression and differentiation. Research also indicates that the S-100 protein may function in the activation of Ca2+ induced Ca2+ release, inhibition of microtubule assembly and inhibition of protein kinase C mediated phosphorylation. Two S-100 subunits, sharing 60% sequence identity, have been described as S-100 α chain and S-100 β chain. Three S-100 dimeric forms have been characterized, differing in their subunit composition of either two α chains, two β chains or one α and one β chain. S-100 localizes to the cytoplasm and nuclei of astrocytes, Schwann's cells, ependymomas and astrogliomas. S-100 is also detected in almost all benign naevi, malignant melanocytic tumours and in Langerhans cells in the skin. Calbindin, S-100 proteins and parvalbumin proteins are each expressed in neural tissues. In addition, S-100 α and β are present in a variety of other tissues, and calbindin is present in intestine and kidney.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: S100 polyclonal antibody detects endogenous levels of S100 protein.

Bioworld Molecular Weight: 11 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of S100 on mouse heart cells lysates using anti-S100 antibody at 1/500 dilution., Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- S100 antibody. Counter stained with hematoxylin.

Alternative Name: S100A1, NEF, Protein S100 B, Protein S100-B, S 100 calcium binding protein beta chain, S 100 protein beta chain, S-100 protein beta chain, S-100 protein subunit beta, S100, S100 calcium binding protein beta (neural), S100 calcium-binding protein B, S100 protein beta chain, S100B, S100B, S100beta,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified