SAE1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91199-50, BS91199-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q9UBE0(Human)
Host: Rabbit
Reactivity: Human
Applications: WB, FC
All Applications: WB:1:500-1:2,000
FC:1:50-1:100
Background: Proteolytic degradation by the ubiquitin (Ub) system is essential for normal cell cycle progression, cellular differentiation and stress responses. Proteins conjugated to Ub are marked for progressive degradation by the 26S Proteasome. AOS-1, also designated SUMO-1-activating enzyme or ubiquitin-like 1-activating enzyme E1A, belongs to the ubiquitin-activating E1 family of proteins and plays an important role in the first step of the UBL1 conjugation pathway. AOS-1, which is a dimeric enzyme, functions as a UBLI E1 ligase, mediating the ATP-dependent activation of UBL1. AOS-1 can bind with UBLE1A and UBLE1B to form a heterodimer which can bind UBL1.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Specificity: SAE1 polyclonal antibody detects endogenous levels of SAE1 protein.
Bioworld Molecular Weight: 38 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of SAE1 on different cell lysates using anti-SAE1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: SiHa
Lane 2: K562
Lane 3: 293, Flow cytometric analysis of A549 cells with SAE1 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Alternative Name: SAE1, AOS1, SUA1, UBLE1A, Activator of SUMO1, AOS1, HSPC140, Sae1, SAE1, Sentrin/SUMO activating protein AOS1, SUA1, SUMO 1 activating enzyme E1 N subunit, SUMO 1 activating enzyme subunit 1, SUMO-activating enzyme subunit 1, Ubiquitin like protein SUMO1 activating enzyme, Ubiquitin-like 1-activating enzyme E1A, UBL E1A, UBLE1A,
Immunogen: Recombinant protein within human SAE1 aa 150-300.
Conjugate: Unconjugated
Modification: Unmodified