Product Description

SAE2 (S621) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1485-50, BS1485-100

Sizes: 50µl, 100µl

Swiss-Prot: Q9UBT2

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: The human UBiquitin Activating domain 2 (UBA2) gene is located on chromosome 19q12. Human UBA2 could form a beta mercaptoethanol-sensitive conjugate with members of the sentrin family, but not with ubiquitin of NEDD8 in the presence of AOS1. The recombinant proteins, Sua1p and hUba2, form a complex. In this complex, hUba2 binds SUMO-1, which gives the complex the activity of the SUMO-1 activating enzyme. Therefore, UBA2 has been found to be a component of the sentrin-activating enzyme complex.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Uba2 (S621) polyclonal antibody detects endogenous levels of Uba2 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of SAE2 (S621) polyclonal antibody at 1:500 dilution
Lane 1: A549 whole cell lysate (40ug)
Lane 2: HEK293T whole cell lysate (40ug)
Lane 3: H1792 whole cell lysate (40ug)
Lane 4: PC12 whole cell lysate (40ug)
Lane 5: BV2 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of SAE2 (S621) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 90 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: SUMO-activating enzyme subunit 2; Anthracycline-associated resistance ARX; Ubiquitin-like 1-activating enzyme E1B; Ubiquitin-like modifier-activating enzyme 2; UBA2; SAE2; UBLE1B; HRIHFB2115

Immunogen: Synthetic peptide, corresponding to amino acids 598-640 of Human SAE2.

Conjugate: Unconjugated

Modification: Unmodified