Product Description

Sam 68 (Y145) Polyclonal Antibody

Catalogue Numbers: BS2952-50, BS2952-100

Sizes: 50µl, 100µl

Alternative Name: KH domain-containing; RNA-binding; signal transduction-associated protein 1; GAP-associated tyrosine phosphoprotein p62; Src-associated in mitosis 68 kDa protein; Sam68; p21 Ras GTPase-activating protein-associated p62; p68; KHDRBS1; SAM68

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q07666

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: SAM68 is recruited and tyrosine phosphorylated by several receptor systems, and once it is phosphorylated, it functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins.It represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. It also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Sam 68 (Y145) polyclonal antibody detects endogenous levels of Sam 68 protein.

Bioworld Molecular Weight: ~ 48 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Sam 68 (Y145) pAb at 1:1000 dilution
Lane 1: HCC827 whole cell lysate(40ug)
Lane 2: Hela whole cell lysate(40ug)
Lane 3: H9C2 whole cell lysate(40ug)
Lane 4: 3T3-L1 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 120-170 of Human Sam 68.

Conjugate: Unconjugated

Modification: Unmodified