Sar1b (F151) Polyclonal Antibody-BS3309

Sar1b (F151) Polyclonal Antibody

Catalogue Numbers: BS3309-50, BS3309-100

Sizes: 50µl, 100µl

Alternative Name: GTP-binding protein SAR1b; GTP-binding protein B; GTBPB; SAR1B; SARA2; SARB

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9Y6B6

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: There are a number of components involved in the secretory pathway of cells. Vesicular traffic within the early secretory pathway is mediated by COPI- and COPII-coated vesicles. The COPII vesicle coat protein promotes the formation of endoplasmic reticulum (ER) derived transport vesicles that carry secretory proteins to the Golgi complex. The SAR1 gene encodes two isoforms, Sar1a and Sar1B, in mammalian cells. These proteins are low-molecular-weight GTPases, which are essential for the formation of transport vesicles from the ER. Mutations in the SAR1 gene result in Anderson’s disease (and/or chylomicron retention disease CMRD), a rare, autosomal recessive lipid malabsorption disorder characterized by chronic diarrhea, failure to thrive and hypocholesterolemia in childhood.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Sar1b (F151) pAb detects endogenous levels of Sar1b protein.

Bioworld Molecular Weight: ~ 22 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Sar1b (F151) pAb at 1:500 dilution
Lane 1: L02 whole cell lysate(40ug)
Lane 2: HCT116 whole cell lysate(40ug)
Lane 3: The Brain tissue lysate of Mouse(40ug)
Lane 4: The Muscle tissue lysate of Rat(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 121-181 of Human Sar1b.

Conjugate: Unconjugated

Modification: Unmodified