Sarcomeric α Actinin Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91206-50, BS91206-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P35609 Human;Q9JI91 Mouse;Entrez Gene: 291245 Rat
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IP, IHC
All Applications: WB:1:500
IP:1:10-1:50
IHC:1:50-1:200
Background: This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status. F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Purification and Purity: Protein A purified.
Storage and Stability: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Specificity: Sarcomeric α Actinin polyclonal antibody detects endogenous levels of Sarcomeric α Actinin protein.
Bioworld Molecular Weight: 104 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Sarcomeric Alpha Actinin on mouse heart tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature., Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Sarcomeric Alpha Actinin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with ET7109-42 at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
Alternative Name: ACTN2, Actin binding protein, Actinin alpha 2, ACTN 2, ACTN2, ACTN2, Alpha actinin 2, Alpha actinin skeletal muscle, Alpha actinin skeletal muscle isoform 2, Alpha-actinin skeletal muscle isoform 2, Alpha-actinin-2, CMD1AA, F actin cross linking protein, F-actin cross-linking protein antibody
Immunogen: Recombinant protein within human Sarcomeric Alpha Actinin aa 1-150.
Conjugate: Unconjugated
Modification: Unmodified