Product Description

SARM1 Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS78063-50, BS78063-100

Swiss-Prot: Q6SZW1

Host: Rabbit

SARM1 Polyclonal Antibody-BS78063

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human

Applications: WB, IP,

All Applications: WB,1:500 - 1:2000 | IP,1:50 - 1:200

Background: NAD(+ hydrolase, which plays a key role in axonal degeneration following injury by regulating NAD(+ metabolism. Acts as a negative regulator of MYD88- and TRIF-dependent toll-like receptor signaling pathway by promoting Wallerian degeneration, an injury-induced form of programmed subcellular death which involves degeneration of an axon distal to the injury site. Wallerian degeneration is triggered by NAD(+ depletion: in response to injury, SARM1 is activated and catalyzes cleavage of NAD(+ into ADP-D-ribose (ADPR, cyclic ADPR (cADPR and nicotinamide; NAD(+ cleavage promoting cytoskeletal degradation and axon destruction. Also able to hydrolyze NADP(+, but not other NAD(+-related molecules. Can activate neuronal cell death in response to stress. Regulates dendritic arborization through the MAPK4-JNK pathway (By similarity. Involved in innate immune response: inhibits both TICAM1/TRIF- and MYD88-dependent activation of JUN/AP-1, TRIF-dependent activation of NF-kappa-B and IRF3, and the phosphorylation of MAPK14/p38.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Unmodification

Bioworld Molecular Weight: 73KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using SARM1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Immunoprecipitation analysis of 300ug extracts of SH-SY5Y cells using 3ug SARM1 antibody. Western blot was performed from the immunoprecipitate using SARM1 antibody at a dilution of 1:1000.

Alternative Name: SARM; HsTIR; SAMD2; hSARM1; MyD88-5

Immunogen: A synthetic peptide of human SARM1(NP_055892.2).

Modification: Unmodified

Conjugate: Unconjugated