SARS-CoV-2 Neutralization Antibody ELISA Kit
Size: 96T
Catalogue Number: E-EL-H0001-96T, E-EL-E620-96T, E-EL-E608-96T
Citations, Manuals and MSDS Available upon request.
Abbreviation: SARS-CoV-2NeutralizationAntibody
Target Synonyms: COVID 19 Neutralization Antibody; Anti-RBD; RBD Antibody; S1RBD Antibody;
Research Areas: SARS-CoV-2, COVID 19
Target Species: Human
Application: ELISA
Usage: This ELISA kit applies to the in vitro Semi-Quantitative determination of SARS-CoV-2 Neutralization Antibody in serum or plasma.
Tested Sample Types: Serum, Plasma
Type: Competitive
Assay Time: 1.5h
Detection Type: Colormetric
Sensitivity: 9.38 ng/mL
Detection Range: 15.63-500 ng/mL
Test Principle: This ELISA kit uses Competitive-ELISA as the method to qualitatively detect the Anti-SARS-CoV-2 Neutralization Antibody in the sample. _x000D_ The micro ELISA plate provided in this kit is pre-coated with recombinant human ACE2. During the reaction, the SARS-CoV-2 Neutralization Antibody in the pretreated samples or controls competes with a fixed amount of human ACE2 on the solid phase supporter for sites on the Horseradish peroxidase (HRP) conjugated recombinant SARS-CoV-2 RBD fragment (HRP-RBD). After 37℃ incubation, the unbound HRP-RBD as well as any HRP-RBD bound to non-Neutralization antibody will be captured on the plate and eventually form the ACE2-RBD-HRP complex, while the circulating neutralization antibodies HRP-RBD complexes remain in the supernatant and are removed during washing. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. Compared with the inhibition ratio to judge whether SARS-CoV-2 Neutralization Antibody exists in the tested samples or not.
Cross Reactivity: This kit recognizes Human SARS-CoV-2 Neutralization Antibody in samples. No significant cross-reactivity or interference between Human SARS-CoV-2 Neutralization Antibody and analogues was observed
Recovery Rate: 80%-120%
Precision: Both intra-CV and inter-CV are < 10%.
Storage: 2-8℃, 12 months
Shipping: ice bag