SARS-CoV-2 NSP16 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS77971-50, BS77971-100
Swiss-Prot: P0DTD1
Host: Rabbit
Reactivity: SARS-CoV-2
Applications: WB
All Applications: WB,1:500 - 1:2000
Background: [Spike protein S1]: attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein. Binding to host NRP1 and NRP2 via C-terminal polybasic sequence enhances virion entry into host cell. This interaction may explain virus tropism of human olfactory epithelium cells, which express high level of NRP1 and NRP2 but low level of ACE2. The stalk domain of S contains three hinges, giving the head unexpected orientational freedom. Uses human TMPRSS2 for priming in human lung cells which is an essential step for viral entry. Can be alternatively processed by host furin. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Unmodification
Extra Notes: Western blot analysis of extracts of normal 293T cells and 293T transfected with NSP16 Protein, using SARS-CoV-2 NSP16 antibody at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Bioworld Molecular Weight: 37KDa
Note: For research use only, not for use in diagnostic procedure.
Immunogen: Recombinant fusion protein of coronavirus NSP16(YP_009724389.1).
Conjugate: Unconjugated
Modification: Unmodified