SARS-CoV-2 NSP3 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS80250-50, BS80250-100
Swiss-Prot: P0DTD1
Host: Rabbit
Reactivity: SARS-CoV-2
Applications: WB
All Applications: WB,1:500 - 1:2000
Background: [Replicase polyprotein 1ab]: Multifunctional protein involved in the transcription and replication of viral RNAs. Contains the proteinases responsible for the cleavages of the polyprotein.
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Unmodification
Extra Notes: Western blot analysis of extracts of normal 293T cells 293T transfected with NSP3 Protein, using SARS-CoV-2 NSP3 antibody at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Bioworld Molecular Weight: 33KDa
Note: For research use only, not for use in diagnostic procedure.
Immunogen: Recombinant fusion protein of coronavirus NSP3(YP_009725299.1).
Conjugate: Unconjugated
Modification: Unmodified