SARS - CoV - 2 NucleoProtein Polyclonal Antibody - BS80224

SARS-CoV-2 NucleoProtein Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS80224-50, BS80224-100

Host: Rabbit

Reactivity: SARS-CoV-2

Applications: WB, IF/ICC, DB, IP

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200 | DB,1:500 - 1:2000 | IP,1:50 - 1:200

Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The nucleocapsid phosphoprotein is a structural protein that binds to, protects the viral RNA genome and is involved in packaging the RNA into virus particles. The N protein has been suggested as an antiviral drug target.

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Extra Notes: Western blot analysis of extracts of normal 293T cells and 293T transfected with Nucleoprotein,using SARS-CoV-2 Nucleoprotein antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Western blot analysis of Recombinant Nucleoprotein of Coronavirus SARS-CoV-2，using SARS-CoV-2 Nucleoprotein antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Immobilized Recombinant SARS-COV-2 Nucleocapsid at 1μg/mL can bind SARS-CoV-2 Nucleoprotein antibody with a linear range of 3.12-200ng/mL., Immunofluorescence analysis of SARS-CoV-2 Nucleoprotein transfect Hela cells using SARS-CoV-2 Nucleoprotein pAb. Blue: DAPI for nuclear staining.

Bioworld Molecular Weight: 50kDa

Note: For research use only, not for use in diagnostic procedure.

Immunogen: Recombinant fusion protein of coronavirus Nucleoprotein(P0DTC9).

Conjugate: Unconjugated

Modification: Unmodified