SARS-CoV-2 ORF7a Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS77978-50, BS77978-100
Swiss-Prot: P0DTC7
Host: Rabbit
Reactivity: SARS-CoV-2
Applications: WB
All Applications: WB,1:500 - 1:2000
Background: Plays a role as antagonist of host tetherin (BST2, disrupting its antiviral effect. Acts by binding to BST2 thereby interfering with its glycosylation. May suppress small interfering RNA (siRNA. May bind to host ITGAL, thereby playing a role in attachment or modulation of leukocytes.
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Unmodification
Extra Notes: Western blot analysis of extracts of normal 293T cells and 293T transfected with ORF7a Protein, using SARS-CoV-2 ORF7a antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Bioworld Molecular Weight: 17KDa
Note: For research use only, not for use in diagnostic procedure.
Immunogen: Recombinant fusion protein of coronavirus ORF7a(YP_009724395.1).
Conjugate: Unconjugated
Modification: Unmodified