SARS-CoV-2 Spike Protein Total Antibody ELISA Kit
Size: 96T
Catalogue Number: E-EL-E620-96T, E-EL-E608-96T, E-EL-E607-96T
Citations, Manuals and MSDS Available upon request.
Abbreviation: SARS-CoV-2 Spike Protein Total Antibody
Target Synonyms: COVID 19 Spike Protein Total Antibody; Spike Protein Total Antibody; Spike Protein Antibody; SP Total Antibody; SP Antibody; SARS-CoV-2 Spike(RBD) Tabć2019-nCoV Spike(RBD) TabćCOVID-19 Spike(RBD) Tab;
Research Areas: SARS-CoV-2, COVID 19
Target Species: Human
Application: ELISA
Usage: This ELISA kit applies to the in vitro Qualitative determination of SARS-CoV-2 Spike Protein Total Antibody in serum or plasma.
Tested Sample Types: Serum, Plasma
Type: Sandwich
Assay Time: 3.5h
Detection Type: Colormetric
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Test Principle: This ELISA kit uses the Sandwich-Ag ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with recombinant SARS-CoV-2 Spike Protein. After adding samples (or controls) to wells, the total antibodies against SARS-CoV-2 Spike Protein will combine with the pre-coated SARS-CoV-2 Spike Protein. After washing completely, add Horseradish Peroxidase (HRP) conjugated SARS-CoV-2 Spike Protein to develop the antigen-antibody-HRP conjugated antigen complex. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SARS-CoV-2 Spike Protein, antibodies against SARS-CoV-2 Spike Protein and HRP conjugated SARS-CoV-2 Spike Protein will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. Compared with the CUT OFF value to judge whether SARS-CoV-2 Spike Protein total antibody exists in the tested samples or not.
Cross Reactivity: This kit recognizes SARS-CoV-2 Spike Protein Total Antibody in samples. No significant cross-reactivity or interference between Human SARS-CoV-2 Neutralization Antibody and analogues was observed
Recovery Rate: 80%-120%
Precision: Intra-CV: CV%ļ¼8%; Inter-CV: CV%ļ¼10%
Storage: 2-8ā/-20ā, 12 months
Shipping: ice bag