Product Description

SATB1 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB11810-50, MB11810-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: Q01826

Host: Rabbit

Reactivity: Human

Applications: WB, IHC-P, IP,

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20

Background: SATB1 is a crucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma (By similarity). Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence (CSBS), at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcriptional repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 86 kDa; Observed MW: 100 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of SATB1 in Hela, A549, HL-60, U2OS lysates using SATB1 antibody., Immunohistochemistry analysis of paraffin-embedded Human tonsil using SATB1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: DNA-binding protein SATB1 (Special AT-rich sequence-binding protein 1)

Immunogen: A synthetic peptide of human SATB1

Conjugate: Unconjugated

Modification: Unmodified