Product Description

SCAMP1 Polyclonal Antibody

Catalogue Numbers: BS5921-50, BS5921-100

Sizes: 50µl, 100µl

Alternative Name: Secretory carrier-associated membrane protein 1; Secretory carrier membrane protein 1; SCAMP

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O15126

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Secretory carrier membrane proteins (SCAMPs) are components of the post Golgi membranes and are invovled in endocytosis, vesicle recycling and membrane trafficking. The structural features of SCAMPs include multiple N-terminal NPF repeats and four highly conserved transmembrane regions. These NPF repeats frequently interact with EH domain proteins and aid in the budding of transport vesicles from the plasma membrane or the Golgi complex. Endocytic budding at the plasma membrane and vesicle budding at the trans-Golgi complex facilitates binding of SCAMP proteins to EH domain proteins. SCAMPs exist as distinct but related proteins that include SCAMP1, SCAMP2, and SCAMP3. Tyrosine-phosphorylation by the epidermal growth factor-receptor of SCAMP1 and SCAMP3 suggests that SCAMPs are regulated by phosphorylation. Although SCAMPs are ubiquitously expressed throughout all tissue, in neural tissue the synaptic vesicles express a particularly high concentration of SCAMP1.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: SCAMP1 polyclonal antibody detects endogenous levels of SCAMP1 protein.

Bioworld Molecular Weight: ~ 37 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of SCAMP1 polyclonal antibody at 1:500 dilution
Lane 1: A549 cell lysate
Lane 2: Raw264.7 cell lysate
Lane 3: PC12 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 293-338 of Human SCAMP1.

Conjugate: Unconjugated

Modification: Unmodified