SCF Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91210-50, BS91210-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P21583(Human)
Host: Rabbit
Reactivity: Human
Applications: WB, ICC/IF, IHC, FC
All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:100-1:500
FC:1:50-1:100
Background: Stem cell factor (SCF) is the ligand for the transmembrane tyrosine kinase receptor proto-oncogene c-Kit. SCF, also designated KL, MGF and SLF, is a pleiotropic cytokine that has two alternatively spliced forms, 248 and 220 amino acids in length in human and mouse systems, respectively. Both the larger form and the smaller form are cleaved to produce soluble forms. The smaller splice variant lacks the proteolytic cleavage site, between amino acids 149 and 177 of the larger SCF form. The larger form of SCF is expressed in fibroblasts, brain and thymus, while the smaller form is found in spleen, testis, placenta and cerebellum. The SCF ligand is essential for the development of germ cells, hematopoietic progenitor cells and melanocyte precursors. SCF can stimulate the proliferation of mature mast cells as well as the maturation and proliferation of immature mast cells.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: SCF polyclonal antibody detects endogenous levels of SCF protein.
Bioworld Molecular Weight: 37 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of SCF on Raji cells lysates using anti-SCF antibody at 1/1,000 dilution., ICC staining SCF in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: MGF, SCF, sKITLG, KITLG, C kit ligand, C-kit ligand, Ckit ligand, DCUA, DFNA69, DKFZp686F2250, familial progressive hyperpigmentation 2, FPH2, FPHH, KIT ligand, Kitl, KITLG, KL 1, KL1, Mast cell growth factor, MGF, MGF stem cell factor, SCF, SCF, SF, SHEP7, sKITLG, Soluble KIT ligand, Steel factor, steel, mouse, homolog of, Stem cell factor, Stem cell factor precursor,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified