Product Description

SCNN1G Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS76119-50, BS76119-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P51170

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF/ICC

All Applications: WB,1:500 - 1:2000 | IHC,1:100 - 1:200 | IF/ICC,1:50 - 1:200

Background: Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the gamma subunit, and mutations in this gene have been associated with Liddle syndrome.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 74kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using SCNN1G antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s., Immunofluorescence analysis of human kidney cancer using SCNN1G antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of mouse kidney using SCNN1G antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: SCNN1G; BESC3; ENaCg; ENaCgamma; PHA1; SCNEG

Immunogen: Recombinant fusion protein of human SCNN1G(NP_001030.2).

Conjugate: Unconjugated

Modification: Unmodified