Product Description

SCNN1G Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS7921-50, BS7921-100

Swiss-Prot: P51170

Host: Rabbit

SCNN1G Polyclonal Antibody-BS7921

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse

Applications: WB, IHC

All Applications: WB,1:500 - 1:1000 | IHC,1:50 - 1:100

Background: Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the gamma subunit, and mutations in this gene have been associated with Liddle syndrome.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 80kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using SCNN1G antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s., Immunohistochemistry of paraffin-embedded mouse lung using SCNN1G antibody at dilution of 1:100.Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Alternative Name: SCNN1G; BESC3; ENaCg; ENaCgamma; PHA1; SCNEG

Immunogen: Recombinant fusion protein of human SCNN1G(NP_001030.2).

Modification: Unmodified

Conjugate: Unconjugated