Product Description

SCXA Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS66161-50, BS66161-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q7RTU7

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: ELISA IHC IF ICC

All Applications: ELISA: 1:5000-1:10000
IHC: 1:100-1:500
IF: 1:100-1:500
ICC: 1:100-1:500

Background: Plays an early essential role in mesoderm formation, as well as a later role in formation of somite-derived chondrogenic lineages.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: SCXA polyclonal antibody detects endogenous levels of SCXA protein.

Bioworld Molecular Weight: ~ 22 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Tissue/cell: rat thyroid gland; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min;Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-SCXA Polyclonal Antibody, Unconjugated(bs-12364R) 1:200, overnight at 4℃.

Alternative Name: Basic helix loop helix transcription factor scleraxis; Basic helix-loop-helix transcription factor scleraxis; bHLHa41; bHLHa48; Class A basic helix-loop-helix protein 41; Class A basic helix-loop-helix protein 48; scleraxis homolog A; SCX; SCX_HUMAN; SCXB

Immunogen: KLH conjugated synthetic peptide derived from human SCXA : 131-201/201

Conjugate: Unconjugated

Modification: Unmodified