Product Description

SDHB Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91215-50, BS91215-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P21912(Human) Q9CQA3(Mouse) P21913(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IHC FC

All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: In aerobic respiration reactions, succinate dehydrogenase (SDH) catalyzes the oxidation of succinate and ubiquinone to fumarate and ubiquinol. Four subunits comprise the SDH protein complex: a flavochrome subunit (SDHA), an iron-sulfur protein (SDHB) and two membrane-bound subunits (SDHC and SDHD) anchored to the inner mitochondrial membrane. Mutations to these subunits cause mitochondrial dysfunction, corresponding to several distinct disorders. Mutations in the membrane bound components may cause hereditary paraganglioma, while SDHA mutations associate with juvenile encephalopathy as well as Leigh syndrome, a severe neurological disorder. Inactivating mutations in SDHB correlate with inherited, but not necessarily sporadic, cases of pheochromocytoma.

Purification and Purity: Protein affinity purified.

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: SDHB polyclonal antibody detects endogenous levels of SDHB protein.

Bioworld Molecular Weight: 32 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of SDHB on different cell lysate using anti-SDHB antibody at 1/1,000 dilution.
Positive control:
Lane 1: human liver
Lane 2: mouse liver
Lane 3: rat liver
Lane 4: rat liver, preincubated with the immunization protein., ICC staining SDHB in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: Ip, SDHB, SDH, SDH1, CWS2, DHSB, FLJ92337, Ip, Iron sulfur subunit, Iron sulfur subunit of complex II, Iron-sulfur subunit of complex II, mitochondrial, PGL 4, PGL4, SDH 1, SDH, SDH1, SDH2, SDH2, homolog of, SdhB, SDHIP, Succinate dehydrogenase [ubiquinone] iron sulfur protein mitochondrial, Succinate dehydrogenase [ubiquinone] iron sulfur subunit, Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial, Succinate Dehydrogenase 1 Iron Sulfur Subunit, Succinate Dehydrogenase 2, S. cerevisiae, homolog of, Succinate dehydrogenase complex iron sulfur subunit B, Succinate dehydrogenase complex subunit B iron sulfur, Succinate Dehydrogenase Complex Subunit B Iron Sulfur Protein, succinate dehydrogenase complex, subunit B, iron sulfur (Ip), Succinate dehydrogenase iron sulfur protein,

Immunogen: Recombinant protein within human SDHB aa 100-300.

Conjugate: Unconjugated

Modification: Unmodified