SELENOM Polyclonal Antibody-BS76894

SELENOM Polyclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: BS76894-50, BS76894-100 Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Swiss-Prot: Q8WWX9 Host: Rabbit Reactivity: Human, Mouse Applications: WB All Applications: WB,1:500 - 1:2000 Background: The protein encoded by this gene belongs to the selenoprotein M/SEP15 family. The exact function of this protein is not known. It is localized in the perinuclear region, is highly expressed in the brain, and may be involved in neurodegenerative disorders. Transgenic mice with targeted deletion of this gene exhibit increased weight gain, suggesting a role for this gene in the regulation of body weight and energy metabolism. This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec). Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity: Unmodification Extra Notes: Western blot analysis of extracts of various cell lines, using SELENOM antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. Note: For research use only, not for use in diagnostic procedure. Bioworld Molecular Weight: 16kDa Alternative Name: SELENOM; SELM; SEPM Immunogen: Recombinant fusion protein of human SELENOM(NP_536355.1). Conjugate: Unconjugated Modification: Unmodified