SEMA4A (N532) Polyclonal Antibody-BS2363

SEMA4A (N532) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2363-50, BS2363-100

Sizes: 50µl, 100µl

Swiss-Prot: Q9H3S1

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: Semaphorins are a family of cell surface and secreted proteins that are conserved from insects to humans. Members of this family of proteins are approximately 750 amino acids in length (including signal sequences) and are defined by a conserved extracellular “Semaphorin” domain of approximately 500 amino acids containing 14-16 cysteines, blocks of conserved sequences and no obvious repeats. The transmembrane semaphorins are characterized by an additional 80 amino acid transmembrane domain and an 80-110 amino acid cytoplasmic domain. Secreted and cell-bound semaphorins chemically attract and repel the growth of neural axons, guiding the development of intricate networks of neural tissue. Semaphorin 4A (SEMA4A), also designated Semaphorin B, is a type I membrane protein. The SEMA4A gene encoding the protein localizes to chromosome 1q22. SEMA4A provides signals to specify territories inaccessible for growing neurons, inhibiting axonal extension.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: SEMA4A (N532) polyclonal antibody detects endogenous levels of SEMA4A protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of SEMA4A (N532) pAb at 1:500 dilution
Lane 1: MCF-7 whole cell lysate (40ug)
Lane 2: K562 whole cell lysate (40ug)
Lane 3: H9C2 whole cell lysate (40ug)
Lane 4: BV2 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of SEMA4A (N532) pAb in paraffin-embedded human brain tissue.

Bioworld Molecular Weight: ~ 84 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Semaphorin-4A; Semaphorin-B; Sema B; SEMA4A; SEMAB; SEMB; UNQ783/PRO1317

Immunogen: Synthetic peptide, corresponding to amino acids 500-546 of Human SEMA4A.

Conjugate: Unconjugated

Modification: Unmodified