Product Description

Separase (A795) Polyclonal Antibody

Catalogue Numbers: AP0374-50, AP0374-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q14674

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB:1:500~1:1000

Background: Separase is a cysteine protease that triggers anaphase in all eukaryotes by participating in separation of sister chromatids during mitosis. Once activated, separase hydrolyzes the SCC1 subunit of cohesin, the chromosomal protein complex responsible for sister chromatid cohesion. Separase and cohesin are highly conserved from yeasts to humans. When the cell is not dividing, separase is prevented from cleaving cohesin through its association with another protein, securin. When anaphase is signaled, the securin is ubiquitinated and hydrolyzed by APC/cyclosome, releasing the active separase. Separase is transiently activated between the two meioses and may also be involved in homolog separation.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Separase (A795) polyclonal antibody detects endogenous levels of Separase protein.

Extra Notes: Western blot (WB) analysis of Separase (A795) pAb at 1:500 dilution
Lane1:EC9706 whole cell lysate(40ug)
Lane2:HEK293T whole cell lysate(40ug)
Lane3:SGC7901 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 230 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Separin; Caspase-like protein ESPL1; Extra spindle poles-like 1 protein; Separase; ESPL1; ESP1; KIAA0165

Immunogen: Synthetic peptide, corresponding to amino acids 763-824 of Human Separase.

Conjugate: Unconjugated

Modification: Unmodified