Product Description

Septin 2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91221-50, BS91221-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q15019(Human) P42208(Mouse) Q91Y81(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IF, IHC FC IP,

All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Filament-forming cytoskeletal GTPase. Forms a filamentous structure with SEPT12, SEPT6, SEPT2 and probably SEPT4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation . Required for normal organization of the actin cytoskeleton. Plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. Required for the progression through mitosis. Forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, may be required for chromosome segregation and spindle elongation. Plays a role in ciliogenesis and collective cell movements. In cilia, required for the integrity of the diffusion barrier at the base of the primary cilium that prevents diffusion of transmembrane proteins between the cilia and plasma membranes: probably acts by regulating the assembly of the tectonic-like complex (also named B9 complex) by localizing TMEM231 protein.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Septin 2 polyclonal antibody detects endogenous levels of Septin 2 protein.

Bioworld Molecular Weight: 41 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Septin 2 on different cell lysates using anti-Septin 2 at 1/500 dilution.
Positive control:
Line 1: Mouse brain
Line 2: SiHa
Line 3: K562, ICC staining Septin 2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: NEDD-5, SEPT2, DIFF6, KIAA0158, NEDD5, DIFF6, hNEDD5, KIAA0158, NEDD 5, NEDD5, Neural precursor cell expressed developmentally down regulated 5, Neural precursor cell expressed developmentally down regulated protein 5, Pnutl3, Protein NEDD5, SEPT2, septin 2,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified