Product Description

SERCA1 (D580) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3934-50, BS3934-100

Sizes: 50µl, 100µl

Swiss-Prot: O14983

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC

Background: The sarcoplasmic reticulum of striated muscle is a specialized intracellular membrane system that plays a critical role in the contraction and relaxation of muscle. The SERCAs mediate Ca2+ uptake into intracellular stores. SERCA-mediated Ca2+ uptake induces and maintains muscular relaxation. The SERCA1 gene is exclusively expressed in type II (fast) skeletal muscle. The SERCA2 gene is subject to tissue-dependent processing which is responsible for the generation of the SERCA2a muscle-specific form expressed in type I (slow) skeletal, cardiac and smooth muscle, and the SERCA2b isoform expressed in all cell types. The SERCA3 gene is not as well characterized and is found in non-muscle cells. SERCA2 plays an important part in regulating cardiac contractile function. SERCA3 is an isoform expressed in several cell types including platelets, lymphoid cells and mast cells. SERCA1, SERCA2 and SERCA3 all undergo alternative splicing.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: SERCA1 (D580) polyclonal antibody detects endogenous levels of SERCA1 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of RIP pAb at 1:500 dilution
Lane 1: HCT116 whole cell lysate (40ug)
Lane 2: CT-26 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of SERCA1 (D580) pAb in paraffin-embedded human skeletal muscle tissue.

Bioworld Molecular Weight: ~ 110 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Sarcoplasmic/endoplasmic reticulum calcium ATPase 1; SERCA1; SR Ca (2+)-ATPase 1; Calcium pump 1; Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform; Endoplasmic reticulum class 1/2 Ca (2+) ATPase; ATP2A1

Immunogen: Synthetic peptide, corresponding to amino acids 551-600 of Human SERCA1.

Conjugate: Unconjugated

Modification: Unmodified