SERCA2 Polyclonal Antibody -BS67171

SERCA2 Polyclonal Antibody

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Catalogue Numbers: BS67171-50, BS67171-100

Sizes: 50µl, 100µl

Swiss-Prot: P16615

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB, IHC

Background: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen (PubMed:16402920, PubMed:12542527).

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 132% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of SERCA2 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of SERCA2 expression in mouse muscle (A), rat muscle (B) whole cell lysates., Immunohistochemical analysis of SERCA2 staining in human muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 114, 130 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: ATP2B; Sarcoplasmic/endoplasmic reticulum calcium ATPase 2; SERCA2; SR Ca (2+)-ATPase 2; Calcium pump 2; Calcium-transporting ATPase sarcoplasmic reticulum type slow twitch skeletal muscle isoform; Endoplasmic reticulum class 1/2 Ca (2+) ATPase

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human SERCA2. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified