SERCA2 Polyclonal Antibody-BS91223

SERCA2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91223-50, BS91223-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P16615(Human) O55143(Mouse) P11507(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, FC

All Applications: WB:1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: ATP dependent calcium pumps are responsible, in part, for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium (SERCA) ATPases. The sarcoplasmic reticulum of striated muscle is a specialized intracellular membrane system that plays a critical role in the contraction and relaxation of muscle. The SERCAs mediate Ca2+ uptake into intracellular stores. SERCA-mediated Ca2+ uptake induces and maintains muscular relaxation. The SERCA1 gene is exclusively expressed in type II (fast) skeletal muscle. The SERCA2 gene is subject to tissue-dependent processing which is responsible for the generation of the SERCA2a muscle-specific form expressed in type I (slow) skeletal, cardiac and smooth muscle, and the SERCA2b isoform expressed in all cell types. The SERCA3 gene is not as well characterized and is found in non-muscle cells. SERCA2 plays an important part in regulating cardiac contractile function. SERCA3 is an isoform expressed in several cell types including platelets, lymphoid cells and mast cells. SERCA1, SERCA2 and SERCA3 all undergo alternative splicing.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: SERCA2 polyclonal antibody detects endogenous levels of SERCA2 protein.

Bioworld Molecular Weight: 115 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: ICC staining SERCA2 in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., ICC staining SERCA2 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: SERCA2, SR Ca(2+)-ATPase 2, ATP2A2, AT2A2, Atp2a2, ATP2B, ATPase Ca++ transporting cardiac muscle slow twitch 2, Calcium pump 2, Calcium-transporting ATPase sarcoplasmic reticulum type, Calcium-transporting ATPase sarcoplasmic reticulum type slow twitch skeletal muscle isoform, Cardiac Ca2+ ATPase, DAR, DD, Endoplasmic reticulum class 1/2 Ca(2+) ATPase, MGC45367, Sarcoplasmic/endoplasmic reticulum calcium ATPase 2, SERCA 2, SERCA2, serca2a, slow twitch skeletal muscle isoform, SR Ca(2+)-ATPase 2,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified