Product Description

SERPINB1 Polyclonal Antibody

Catalogue Numbers: BS62099-50, BS62099-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: P30740

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The serine proteinase inhibitors (serpins) compose a superfamily of proteins with a diverse set of functions, including the control of blood coagulation, complement activation, programmed cell death and development. Serpins are secreted glycoproteins that contain a stretch of peptide that mimics a true substrate for a corresponding serine protease. The monocyte/neutrophil elastase inhibitor gene, SERPINB1, belongs to the Ov-serpin family (ovalbuminrelated serpins). Human SerpinB1, also designated monocyte/neutrophil elastase inhibitor (M/NEI) or leukocyte elastase inhibitor (LEI), is a cytoplasmic protein which acts as a fast-acting stoichiometric proteinase inhibitor that regulates the activity of neutrophil elastase (NE), cathepsin-G and proteinase-3. There are four homologous genes in mouse designated SerpinB1a, SerpinB1b, SerpinB1c and the pseudogene, Serpinb1-ps1. The three protein-coding genes share significant sequence identity, however SerpinB1a (also designated EIA) has been characterized as the functional ortholog of human SerpinB1.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: SERPINB1 polyclonal antibody detects endogenous levels of SERPINB1 protein.

Extra Notes: Western blot (WB) analysis of SERPINB1 polyclonal antibody at 1:500 dilution
Lane1:K562 whole cell lysate(40ug)
Lane2:The Spleen tissue lysate of Rat(40ug)
Lane3:The Spleen tissue lysate of Mouse(40ug)
Lane4:Myla2059 whole cell lysate(40ug)
Lane5:Jurkat whole cell lysate(40ug)
Lane6:A549 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 47 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Leukocyte elastase inhibitor; LEI; Monocyte/neutrophil elastase inhibitor; EI; M/NEI; Peptidase inhibitor 2; PI-2; Serpin B1; SERPINB1; ELANH2; MNEI; PI2

Immunogen: Synthetic peptide, corresponding to Human SERPINB1.

Conjugate: Unconjugated

Modification: Unmodified