Product Description

SF1 Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

Catalogue Numbers: GCP58-50, GCP58-100

Sizes: 50µl, 100µl

Swiss-Prot: Q15637

Host: Rabbit

Reactivity: Human

Applications: WB, IHC

Background: Mammalian splicing factor 1 (designated SF1, zinc finger protein 162, ZFM1,CW17R and mammalian branch point binding protein [mBBP]) specificallyrecognizes the seven-nucleotide branch point sequence located at 3' splicesites and participates in the assembly of early spliceosomal complexes.Splicing factor 1 functions as a transcriptional repressor and may control bothproliferation and expression of pro-inflammatory gene products in smoothmuscle cells. In addition, cytokine-induced downregulation of splicing factor 1 expression may contribute to the pathogenesis of hyperproliferative inflammatory diseases. The structure of splicing factor 1 contains a nuclear transport domain, a metal binding motif, and glutamine- and proline-rich regions.Human splicing factor 1 also exists as several different isoforms, H1-isoformand Bo-isoform, produced by alternative splicing events. The human splicingfactor 1 gene is located on chromosome 11 close to the gene encoding Menin,the gene responsible for multiple endocrine neoplasia-type 1 (MEN1).

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: SF1 polyclonal antibody detects endogenous levels of SF1 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of SF1 polyclonal antibody at 1:1000 dilution
Lane 1: A549 whole cell lysate (40ug)
Lane 2: A2780 whole cell lysate (40ug)
Lane 3: HEK293T whole cell lysate (40ug), Immunohistochemistry of paraffin-embedded Rat Brain using SF1 antibody at dilution of 1:50.

Bioworld Molecular Weight: ~ 75 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Splicing factor 1; Mammalian branch point-binding protein; BBP; mBBP; Transcription factor ZFM1; Zinc finger gene in MEN1 locus; Zinc finger protein 162; SF1; ZFM1; ZNF162

Immunogen: Synthetic peptide, corresponding to Human SF1.

Conjugate: Unconjugated

Modification: Unmodified