SF2 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91228-50, BS91228-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q07955(Human) Q6PDM2(Mouse)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC, IF, IHC, FC
All Applications: WB:1:500-1:1,000
ICC:1:50
IHC:1:50-1:200
FC:1:50-1:100
Background: Pre-mRNA splicing enhancer elements are short RNA sequences capable of activating weak splice sites in nearby introns that are required for accurate splice site recognition and the control of alternative splicing. Splicing enhancer elements contain specific binding sites for serine/arginine (SR)-rich splicing factors, which include SC35, 9G8, SRp20, and SF2/ASF. The family of SR factors all contain one or more RNA recognition motifs (RRM) and an arginine/ serine (RS)-rich domain. They are not only essential for constitutive splicing but also regulate splicing in a concentration-dependent manner by influencing the selection of alternative splice sites. The majority of SR proteins, including SC35 and SRp40, are confined to the nucleus, while SF2/ASF, SRp20, and 9G8 are continuously shuttled between the nucleus and the cytoplasm and contribute to mRNA transport. The activity of SR proteins in regulated splicing is antagonized by members of the hnRNP A/B family of proteins, which induce drastic shifts in the selection of splicing sites.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: SF2 polyclonal antibody detects endogenous levels of SF2 protein.
Immunogen: Recombinant protein
Conjugate: Unconjugated
Modification: Unmodified
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of SF2 on different lysates using anti-SF2 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse heart
Lane 2: Mouse liver
Lane 3: K562, ICC staining SF2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.