Product Description

SF3B1 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB12244-50, MB12244-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: O75533

Host: Rabbit

Reactivity: Human, Mouse, Hamster, Rat

Applications: WB, IHC-F, IHC-P, ICC/IF

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200

Background: SF3B1 is subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 146 kDa; Observed MW: 155 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunocytochemistry analysis of SF3B1 in 293T using SF3B1 antibody, and DAPI., Western blot analysis of SF3B1 in 3T3 lysates using SF3B1 antibody., Western blot analysis of SF3B1 in Hela, CHO-K1, C6, Ramos lysates using SF3B1 antibody, Immunohistochemistry analysis of paraffin-embedded Human lung cancer using SF3B1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: SF3B1; SAP155; Splicing factor 3B subunit 1; Pre-mRNA-splicing factor SF3b 155 kDa subunit; SF3b155; Spliceosome-associated protein 155; SAP 155

Immunogen: A synthetic peptide of human SF3B1

Conjugate: Unconjugated

Modification: Unmodified