Product Description

SF3B1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91229-50, BS91229-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O75533(Human) Q99NB9(Mouse)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC ICC IF FC

All Applications: WB:1:500-1:2,000
IHC:1:50-1:200
ICC:1:50
FC:1:50-1:200

Background: Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: SF3B1 polyclonal antibody detects endogenous levels of SF3B1 protein.

Bioworld Molecular Weight: 146 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of SF3B1 on different lysates using anti-SF3B1 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse thymus
Lane 2: Hela, Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-SF3B1 antibody. Counter stained with hematoxylin.

Alternative Name: SF3b155, SAP 155, SF3B1, SAP155, Hsh 155, MDS, Pre mRNA processing 10, Pre mRNA splicing factor SF3b 155 kDa subunit, PRP 10, PRP10, PRPF 10, PRPF10, SAP 155, SAP155, SF3B 1, SF3b155, Spliceosome associated protein 155, Splicing factor 3b subunit 1 155kDa, Splicing factor 3B subunit 1,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified